N1408

Sigma

 

NGF-β human

recombinant, expressed in mouse NSO cells, lyophilized powder, cell culture tested, from human

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Description

Analysis NoteThe biological activity is measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1.
Physical formLyophilized from 0.2% acetic acid containing 5 mg bovine serum albumin.

Properties

biological source from human
recombinantexpressed in mouse NSO cells
assay>97% (SDS-PAGE)
formlyophilized powder
potency0.15-3.0 ng/mL ED50/EC50
total impurities Endotoxin, tested
suitabilitycell culture tested
storage temp.−20°C

Safety

WGK Germany3

References

referenceKorsching, S., The neurotrophic factor concept: A re-examination. J. Neurosci. 13, 2739-2748, (1993) Abstract
 Crutcher, K.A., Detection of NGF-like activity in human brain tissue: Increased levels in Alzheimer's disease. J. Neurosci. 13, 2540, (1993) Abstract
 Fukumoto,H., et al., Recombinant human basic fibroblast growth factor (rhbFGF) induces secretion of nerve growth factor (NGF) in cultured rat astroglial cells. Neurosci. Lett. 122, 221-224, (1991) Abstract