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N1408
Sigma
NGF-β human
recombinant, expressed in mouse NSO cells, lyophilized powder, cell culture tested, from human
Description
| Analysis Note | The biological activity is measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1. |
| Physical form | Lyophilized from 0.2% acetic acid containing 5 mg bovine serum albumin. |
Properties
| biological source | from human |
| recombinant | expressed in mouse NSO cells |
| assay | >97% (SDS-PAGE) |
| form | lyophilized powder |
| potency | 0.15-3.0 ng/mL ED50/EC50 |
| total impurities | Endotoxin, tested |
| suitability | cell culture tested |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| reference | Korsching, S., The neurotrophic factor concept: A re-examination. J. Neurosci. 13, 2739-2748, (1993) Abstract |
| Crutcher, K.A., Detection of NGF-like activity in human brain tissue: Increased levels in Alzheimer's disease. J. Neurosci. 13, 2540, (1993) Abstract | |
| Fukumoto,H., et al., Recombinant human basic fibroblast growth factor (rhbFGF) induces secretion of nerve growth factor (NGF) in cultured rat astroglial cells. Neurosci. Lett. 122, 221-224, (1991) Abstract |




